Filtering to Show DNA and RNA Separately in Slides Stained for Both Nucleic Acids

Design, synthesis and DNA/RNA binding studies of nucleic acids comprising stereoregular and acyclic polycarbamate backbone: polycarbamate nucleic acids (PCNA).

The designed, chiral, acyclic polycarbamate nucleic acids (PCNA) exhibited sequence and orientation specific binding to nucleic acids. Complexes of PCNA with DNA were as stable as PNA:DNA complexes and those with RNA were as stable as natural DNA:RNA complexes.

Magnetic peptide nucleic acids for DNA targeting.

A versatile synthetic platform for the efficient immobilization of PNAs on magnetic iron oxides, providing magnetic nanosensors for selective DNA recognition, is presented.

Quantification of polysaccharides fixed to Gram stained slides

I discovered indigo rings and circles in ATCC® 25922TM Escherichia coli cultures when I added the non-specific polysaccharide stain lactophenol cotton blue to Gram stained slides sampled from 96-well plates used to measure quantitative growth kinetics (QGK) in virtual colony count antimicrobial assays. I attribute the dark blue staining to the presence of capsular polysaccharides and bacterial ...

Analysis of nucleic acids in Daphnia: development of methods and ontogenetic variations in RNA-DNA content

Estimation of RNA concentration or total RNA content is an increasingly widespread method for assessing growth and production rate of an organism in both field and laboratory settings. The basis for an overall association between growth and RNA concentration is: (i) RNA makes up 50–60% of the ribosome, the machine of protein synthesis and subsequent cell growth (Becker, 1986), and (ii) this rib...

All-atom empirical force field for nucleic acids: II. Application to molecular dynamics simulations of DNA and RNA in solution